Identification of 21 genes of infectious laryngotracheitis virus using random sequencing of genomic DNA.

Replication Kinetic of Infectious Laryngotracheitis Virus in Embryonic Chicken Neural Stem Cell

Background and Aims: Infectious laryngotracheitis virus (ILTV) infection causes a highly contagious respiratory disease in poultry leads to significant economic losses in the poultry industry. The genomic and antigenic characteristics between virulent and vaccine strains of ILTV are very similar. The ability of embryonated chicken neural stem (CNS) cell for differential diagnosis of ILTV strain...

Differentiation of field isolates and vaccine strains of infectious laryngotracheitis virus by DNA sequencing.

Two different regions of the infected cell protein 4 (ICP4) gene of infectious laryngotracheitis virus (ILTV) were amplified and sequenced for characterization of field isolates and tissue culture-origin (TCO) and chicken embryo-origin (CEO) vaccine strains. Phylogenetic analysis of the two regions showed differences in nucleotide and amino acid sequences between field isolates and attenuated v...

Studies on the immunogenic proprties of a Live Infectious Laryngotracheitis virus vaccine

Detection of infectious laryngotracheitis virus (Gallid herpesvirus-1) from clinically infected chickens in Egypt by different diagnostic methods

Infectious laryngotracheitis (ILT) disease is an acute highly contagious viral disease leading to massive economic losses to the national poultry industry. This study aimed to identify the most accurate and rapid diagnostic methods to rescue layer poultry farms from intense outbreaks in Egypt. Fifty pathological specimens were collected and subjected to virus isolation (VI), histopathology, dir...

Methylome Analysis in Chickens Immunized with Infectious Laryngotracheitis Vaccine

In this study we investigated the methylome of chickens immunized with Infectious laryngotracheitis (ILT) vaccine derived from chicken embryos. Methyl-CpG binding domain protein-enriched genome sequencing (MBD-Seq) method was employed in the detection of the 1,155 differentially methylated regions (DMRs) across the entire genome. After validation, we ascertained the genomic DMRs distribution an...